Journal: iScience

Article Title: Intestinal microbiota-associated LysoPC(16:0) promotes endometriosis via PPARγ-mediated M2 macrophage polarization and IL-10 secretion

doi: 10.1016/j.isci.2026.117917

Figure Lengend Snippet: Peritoneal macrophages and IL-10 are required for LysoPC(16:0)-driven EM progression (A) Representative lesion photographs from CL- and EL-treated mice, photographed alongside a graduated scale board (cm/mm divisions). (B) Lesion weight; unpaired Student’s t test. (C) Proportion of F4/80 + cells in peritoneal lavage fluid; unpaired Welch’s t test. (D) Representative flow cytometry plots of F4/80 + cells in peritoneal lavage fluid from CL- and EL-treated mice. (E) RT-qPCR of Il10 mRNA in BMDMs stimulated with NC, IL-4, IL-4 + LysoPC(16:0), or IL-4 + LysoPC(16:0) + GW9662; ordinary one-way ANOVA with Šídák’s multiple comparisons test among the IL-4, IL-4 + LysoPC(16:0), and IL-4 + LysoPC(16:0) + GW9662 groups. (F) Representative lesion photographs from anti-IL-10- and isotype control-treated mice, photographed alongside a graduated scale board (cm/mm divisions). (G) Lesion weight; unpaired Student’s t test. n = 6 mice per group (A–D, F, and G); n = 3 technical replicates from 3 biological replicates (E); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; ns, not significant. Data are represented as mean ± SD (B, E, and G) and mean ± SEM (C).

Article Snippet: Anti-mouse IL-10 neutralizing antibody , MedChemExpress , Cat#HY-P990001.

Techniques: Flow Cytometry, Quantitative RT-PCR, Control

Journal: iScience

Article Title: Intestinal microbiota-associated LysoPC(16:0) promotes endometriosis via PPARγ-mediated M2 macrophage polarization and IL-10 secretion

doi: 10.1016/j.isci.2026.117917

Figure Lengend Snippet: The LysoPC(16:0)-PPARγ-M2-IL-10 axis promotes migration of human primary ESCs (A) Proportion of CD206 + cells in THP-1-derived macrophages stimulated under four conditions (NC, IL-4/IL-13, IL-4/IL-13 + LysoPC(16:0), and IL-4/IL-13 + LysoPC(16:0) + GW9662); ordinary one-way ANOVA with Šídák’s multiple comparisons test among sequential conditions. (B) Representative flow cytometry plots of CD206 + THP-1-derived macrophages under the four stimulation conditions. (C) Quantification of migrated ESCs from five patients under each of six conditioned medium conditions; ordinary one-way ANOVA with Šídák’s multiple comparisons test against the NC group. (D) Representative crystal violet-stained migration images of human primary ESCs exposed to conditioned medium from THP-1 macrophages under six stimulation conditions; scale bars, 300 μm. n = 3 technical replicates from 3 biological replicates (A); n = 5 patients (C); ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001. Data are represented as mean ± SD.

Article Snippet: Anti-mouse IL-10 neutralizing antibody , MedChemExpress , Cat#HY-P990001.

Techniques: Migration, Derivative Assay, Flow Cytometry, Staining

Journal: iScience

Article Title: Intestinal microbiota-associated LysoPC(16:0) promotes endometriosis via PPARγ-mediated M2 macrophage polarization and IL-10 secretion

doi: 10.1016/j.isci.2026.117917

Figure Lengend Snippet:

Article Snippet: Anti-mouse IL-10 neutralizing antibody , MedChemExpress , Cat#HY-P990001.

Techniques:

Journal: iScience

Article Title: Intestinal microbiota-associated LysoPC(16:0) promotes endometriosis via PPARγ-mediated M2 macrophage polarization and IL-10 secretion

doi: 10.1016/j.isci.2026.117917

Figure Lengend Snippet:

Article Snippet: Anti-mouse IL-10 neutralizing antibody , MedChemExpress , Cat#HY-P990001.

Techniques: